Raman spectroscopy predicts the link between claw keratin and bone collagen structure in a rodent model of oestrogen deficiency

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1864

Article

Caraher, M. Clare^1,12  Sophocleous, Antonia^2,10  Beattie, J. Renwick³  O'Driscoll, Olive⁴  Cummins, Niarnh M.⁵  Brennan, Orlaith^6,7,8  O'Brien, Fergal J.^6,7,8  Ralston, Stuart H.²  Bell, Steven E. J.¹  Towler, Mark⁹  Idris, Aymen I.^2,11 

[1] Queens Univ Belfast, Sch Chem & Chem Engn, Stranmillis Rd, Belfast, Antrim, North Ireland

[2] Univ Edinburgh, MRC Inst Genet & Mol Med, Western Gen Hosp, Rheumatol & Bone Dis Unit,Ctr Genom & Expt Med, Edinburgh, Midlothian, Scotland

[3] Causeway Enterprise Agcy, J Renwick Beattie Consulting, Ballycastle, North Ireland

[4] Rubicon Ctr, AventaMed, Rossa Ave, Cork, Ireland

[5] Univ Limerick, Grad Entry Med Sch, Ctr Intervent Infect Inflammat & Immun, Limerick, Ireland

[6] Royal Coll Surgeons Ireland, Dept Anat, Tissue Engn Res Grp, Dublin, Ireland

[7] Trinity Coll Dublin, Trinity Ctr Bioengn, Dublin, Ireland

[8] TCD, Adv Mat & Bioengn Res Ctr AMBER, RCSI, Dublin, Ireland

[9] Ryerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada

[10] European Univ Cyprus, Dept Life Sci, Nicosia, Cyprus

[11] Univ Sheffield, Med Sch, Dept Oncol & Metab, Beech Hill Rd, Sheffield, S Yorkshire, England

[12] ICON Plc, South Cty Business Pk, Dublin, Ireland

关键词: Raman spectroscopy;    Bone;    Collagen;    Keratin;    Osteoporosis;    microCT;   

DOI  :  10.1016/j.bbadis.2017.10.020

来源: Elsevier

【 摘 要 】

Osteoporosis is a common disease characterised by reduced bone mass and an increased risk of fragility fractures. Low bone mineral density is known to significantly increase the risk of osteoporotic fractures, however, the majority of non-traumatic fractures occur in individuals with a bone mineral density too high to be classified as osteoporotic. Therefore, there is an urgent need to investigate aspects of bone health, other than bone mass, that can predict the risk of fracture. Here, we successfully predicted association between bone collagen and nail keratin in relation to bone loss due to oestrogen deficiency using Raman spectroscopy. Raman signal signature successfully discriminated between ovariectomised rats and their sham controls with a high degree of accuracy for the bone (sensitivity 89%, specificity 91%) and claw tissue (sensitivity 89%, specificity 82%). When tested in an independent set of claw samples the classifier gave 92% sensitivity and 85% specificity. Comparison of the spectral changes occurring in the bone tissue with the changes occurring in the keratin showed a number of common features that could be attributed to common changes in the structure of bone collagen and claw keratin. This study established that systemic oestrogen deficiency mediates parallel structural changes in both the claw (primarily keratin) and bone proteins (primarily collagen). This strengthens the hypothesis that nail keratin can act as a surrogate marker of bone protein status where systemic processes induce changes.

【 授权许可】

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