SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles

TALANTA

卷:236

Article

Aithal, Srivatsa¹  Mishriki, Sarah²  Gupta, Rohit¹  Sahu, Rakesh P.^1,2,3  Botos, George^4,5  Tanvir, Shazia⁴  Hanson, Russell W.⁵  Puri, Ishwar K.^1,2,3 

[1] McMaster Univ, Dept Mech Engn, Hamilton, ON, Canada

[2] McMaster Univ, Sch Biomed Engn, Hamilton, ON, Canada

[3] McMaster Univ, Dept Mat Sci & Engn, Hamilton, ON, Canada

[4] Genemis Labs, Cambridge, ON, Canada

[5] Aptavid, New York, NY USA

关键词: SARS-CoV-2;    Spike protein;    Aptamer;    Biosensing;    Surface plasmon resonance;    Gold nanoparticle;   

DOI  :  10.1016/j.talanta.2021.122841

来源: Elsevier

【 摘 要 】

A rapid detection test for SARS-CoV-2 is urgently required to monitor virus spread and containment. Here, we describe a test that uses nanoprobes, which are gold nanoparticles functionalized with an aptamer specific to the spike membrane protein of SARS-CoV-2. An enzyme-linked immunosorbent assay confirms aptamer binding with the spike protein on gold surfaces. Protein recognition occurs by adding a coagulant, where nanoprobes with no bound protein agglomerate while those with sufficient bound protein do not. Using plasmon absorbance spectra, the nanoprobes detect 16 nM and higher concentrations of spike protein in phosphate-buffered saline. The timevarying light absorbance is examined at 540 nm to determine the critical coagulant concentration required to agglomerates the nanoprobes, which depends on the protein concentration. This approach detects 3540 genome copies/mu l of inactivated SARS-CoV-2.

【 授权许可】

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