Molecularly imprinted electrochemical sensor for the point-of-care detection of a breast cancer biomarker (CA 15-3)

SENSORS AND ACTUATORS B-CHEMICAL

卷:256

Article

Pacheco, Joao G.¹  Silva, Marta S. V.¹  Freitas, Maria¹  Nouws, Henri P. A.¹  Delerue-Matos, Cristina¹ 

[1] Politecn Porto, Inst Super Engn Porto, REQUIMTE LAQV, Rua Dr Antonio Bernardino de Almeida 431, P-4200072 Oporto, Portugal

关键词: Breast cancer;    Molecularly imprinted polymer;    CA 15-3;    Electrochemical sensor;    Point-of-care analysis;   

DOI  :  10.1016/j.snb.2017.10.027

来源: Elsevier

【 摘 要 】

The incidence of breast cancer has been increasing over the years. To control and monitor this disease several tumor biomarkers have been proposed for early diagnosis, patient follow-up and/or treatment guidance. The only serum breast cancer biomarker in current use is the cancer antigen 15-3 (CA 15-3). In this work a molecularly imprinted polymer (MIP)-based electrochemical (voltammetric) sensor to monitor breast cancer was developed, based on direct surface imprinting of CA 15-3 on a screen-printed gold electrode (Au-SPE). The imprinting was performed in two steps: (1) adsorption of CA 15-3 on the surface of the Au-SPE and (2) electropolymerization of 2-aminophenol around the adsorbed protein. After extraction of the imprinted protein voltammetric analysis was conducted using hexacyanoferrate(II/III) as redox probe, measuring the signals before and after protein binding. The sensor was characterized by voltammetric techniques and electrochemical impedance spectroscopy, and the analytical responses of imprinted and non-imprinted polymer sensors were studied. A linear relationship between the peak current intensity of the redox probe and the logarithm of CA15-3 concentration was established between 5 and 50 UmL(-1), achieving a limit of detection of 1.5 UmL(-1). The prepared MIP-sensor provides fast (15 min) analysis and is cheap, easy to prepare, disposable and could easily be integrated in small portable point-of care devices. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

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