Low impedance and highly transparent microelectrode arrays (MEA) for in vitro neuron electrical activity probing

SENSORS AND ACTUATORS B-CHEMICAL

卷:327

Article

Susloparova, Anna¹  Halliez, Sophie²  Begard, Severine²  Colin, Morvane²  Buee, Luc²  Pecqueur, Sebastien¹  Alibart, Fabien^1,3  Thomy, Vincent¹  Arscott, Steve¹  Pallecchi, Emiliano¹  Coffinier, Yannick¹ 

[1] Univ Lille, Univ Polytech Hauts de France, CNRS, Cent Lille,UMR 8520,IEMN, F-59000 Lille, France

[2] Univ Lille, U1172 LilNCog Lille Neurosci & Cognit, CHU Lille, INSERM, F-59000 Lille, France

[3] Univ Sherbrooke, Lab Nanotechnol & Nanosyst LN2, CNRS, Sherbrooke, PQ J1X 0A5, Canada

关键词: Microelectrode array;    Transparent electrodes;    PEDOT:PSS;    Low impedance;    Neuronal network activity;   

DOI  :  10.1016/j.snb.2020.128895

来源: Elsevier

【 摘 要 】

In this study, we present the microfabrication and characterization of a transparent microelectrode array (MEA) system based on PEDOT:PSS for electrophysiology. The influence of the PEDOT:PSS electrode dimensions on the impedance was investigated and the stability over time under physiological environment was demonstrated. A very good transparency value was obtained by our system displaying one of the best impedance and transmittance values when compared to other transparent MEA. After biocompatibility validation, we successfully recorded spontaneous neuronal activity of primary cortical neurons cultured over 4 weeks on the transparent PEDOT:PSS electrodes. This work shows that microelectrodes composed of PEDOT:PSS are very promising as a new tool for both electrophysiology and fluorescence microscopy studies on neuronal cell cultures.

【 授权许可】

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