A simple and rapid fluorescent aptasensor for ultrasensitive detection of arsenic based on target-induced conformational change of complementary strand of aptamer and silica nanoparticles

SENSORS AND ACTUATORS B-CHEMICAL

卷:256

Article

Taghdisi, Seyed Mohammad^1,2  Danesh, Noor Mohammad^3,4  Ramezani, Mohammad³  Emrani, Ahmad Sarreshtehdar⁵  Abnous, Khalil^6,7 

[1] Mashhad Univ Med Sci, Targeted Drug Delivery Res Ctr, Mashhad, Iran

[2] Mashhad Univ Med Sci, Sch Pharm, Dept Pharmaceut Biotechnol, Mashhad, Iran

[3] Mashhad Univ Med Sci, Nanotechnol Res Ctr, Mashhad, Iran

[4] Res Inst Sci & New Technol, Mashhad, Iran

[5] Mashhad Univ Med Sci, Cardiovasc Res Ctr, Fac Med, Mashhad, Iran

[6] Mashhad Univ Med Sci, Pharmaceut Res Ctr, Mashhad, Iran

[7] Mashhad Univ Med Sci, Sch Pharm, Dept Med Chem, Mashhad, Iran

关键词: Arsenic;    Fluorescent aptasensor;    Conformational change;    Silica nanoparticles;   

DOI  :  10.1016/j.snb.2017.10.129

来源: Elsevier

【 摘 要 】

Simple and reliable sensors for detection of arsenic (As), a toxic heavy metal, in drinking water and blood serum are highly desirable. Herein, we reported the fabrication and characterization of a fluorescent aptasensor for the detection of As (III) using target-induced conformational change of the Biotin and FAM-labeled complementary strand of aptamer (CS1), silica nanoparticles coated with streptavidin (SNPs-Streptavidin) and a label-free aptamer (Apt). The sensor described here had several attractive features, such as simplicity, rapid response and use of label-free Apt. Upon addition of As (III), Apt released its CSs and CS1 formed a hairpin structure on the surface of SNPs-Streptavidin, leading to a strong fluorescence signal. Without introduction of As (III), Apt was hybridized with its CSs and a weak fluorescence signal was obtained. The sensor exhibited a wide linear range between 2 and 500 nM and a very low detection limit of 0.45 nM. In addition, the applicability of the developed method was tested using spiked tap water and serum samples with satisfactory results. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

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