Human mesenchymal stem cells labelled with dye-loaded amorphous silica nanoparticles: long-term biosafety, stemness preservation and traceability in the beating heart

Journal of Nanobiotechnology

Claudia Giachino¹  Valentina Turinetto¹  Pasquale Pagliaro¹  Claudia Penna¹  Gianmario Martra²  Francesca Tullio¹  Federico Catalano²  Lisa Accomasso¹  Silvia Saviozzi¹  Tânia Capelôa¹  Clara Gallina¹ 

[1] Department of Clinical and Biological Sciences, University of Turin, 10, Regione Gonzole, Orbassano, CAP 10043, TO, Italy;Department of Chemistry, Interdepartmental Centre “Nanostructured Interfaces and Surfaces”, University of Turin, 7, Via P. Giuria, Turin, CAP 10125, Italy

关键词: Heart;    Stem cell tracking;    Toxicity;    Silica nanoparticles;    Mesenchymal stem cells;   

Others  :  1231694
DOI  :  10.1186/s12951-015-0141-1

 received in 2015-09-11, accepted in 2015-10-22,  发布年份 2015

【 摘 要 】

Background

Treatment of myocardial infarction with mesenchymal stem cells (MSCs) has proven beneficial effects in both animal and clinical studies. Engineered silica nanoparticles (SiO ₂ -NPs) have been extensively used as contrast agents in regenerative medicine, due to their resistance to degradation and ease of functionalization. However, there are still controversies on their effective biosafety on cellular systems. In this perspective, the aims of the present study are: 1) to deeply investigate the impact of amorphous 50 nm SiO ₂ -NPs on viability and function of human bone marrow-derived MSCs (hMSCs); 2) to optimize a protocol of harmless hMSCs labelling and test its feasibility in a beating heart model.

Results

Optimal cell labelling is obtained after 16 h exposure of hMSCs to fluorescent 50 nm SiO ₂ -NPs (50 µg mL ⁻¹ ); interestingly, lysosomal activation consequent to NPs storage is not associated to oxidative stress. During prolonged culture hMSCs do not undergo cyto- or genotoxicity, preserve their proliferative potential and their stemness/differentiation properties. Finally, the bright fluorescence emitted by internalized SiO ₂ -NPs allows both clear visualization of hMSCs in normal and infarcted rat hearts and ultrastructural analysis of cell engraftment inside myocardial tissue.

Conclusions

Overall, 50 nm SiO ₂ -NPs display elevated compatibility with hMSCs in terms of lack of cyto- and genotoxicity and maintenance of important features of these cells. The demonstrated biosafety, combined with proper cell labelling and visualization in histological sections, make these SiO ₂ -NPs optimal candidates for the purpose of stem cell tracking inside heart tissue.

【 授权许可】

   
2015 Gallina et al.

【 图 表 】

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