Nano-plate biosensor array using ultrafast heat transport through proteins

SENSORS AND ACTUATORS B-CHEMICAL

卷:278

Article

Ogi, Hirotsugu¹  Iwagami, Shigeru²  Nagakubo, Akira¹  Taniguchi, Takuya³  Ono, Teruo⁴ 

[1] Osaka Univ, Grad Sch Engn, Yamadaoka 2-1, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Engn Sci, Machikaneyama 1-3, Toyonaka, Osaka 5608531, Japan

[3] Tech Univ Munich, Phys Dept, Garching, Germany

[4] Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan

关键词: Label-free biosensor;    Pump-probe laser measurement;    Thermoreflectance measurement;    MEMS;    Multichannel;   

DOI  :  10.1016/j.snb.2018.09.030

来源: Elsevier

【 摘 要 】

A novel label-free biosensor array is proposed, which uses the ultrafast heat transport from a metallic nano-plate into surrounding solution across proteins captured on the nano-plate. Using electron-beam lithography, 2500 gold nano-plates of 30 nm thick with 5 x 5 mu m(2) area were fabricated on a fused silica substrate, on which receptor proteins were immobilized. Thermal phonons were induced on the back surface of a single nano-plate by focusing ultrafast light pulses from the substrate side, which propagated toward surrounding solution through surface proteins. Because the ultrafast heat-transport behavior, which is completed within similar to 1 ns, is affected by existence of target proteins on the nano-plate, each nano-plate works as a single label-free biosensor. A theoretical calculation was made for predicting the cooling-down behavior of the nano-plate after an impulsive heating, and a pump-probe optics was developed for monitoring the ultrafast temperature change after excitation with a femtosecond fiber laser. We then confirm that each nano-plate acts as a single label-free biosensor by performing antigen-antibody binding reactions, indicating that a large scale number of multichannel measurement is made possible with this phenomenon.

【 授权许可】

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