Nanoparticle transfer biosensors for the non-invasive detection of SARS-CoV-2 antigens trapped in surgical face masks

SENSORS AND ACTUATORS B-CHEMICAL

卷:345

Article

Vaquer, Andreu¹  Alba-Patino, Alejandra^1,6  Adrover-Jaume, Cristina^1,6  Russell, Steven M.¹  Aranda, Maria^1,2  Borges, Marcio^1,2  Mena, Joana^1,2  del Castillo, Alberto^1,2  Socias, Antonia^1,2  Martin, Luisa^1,3  Magdalena Arellano, Maria^1,3  Agudo, Miguel^1,3  Gonzalez-Freire, Marta⁴  Besalduch, Manuela⁵  Clemente, Antonio¹  Baron, Enrique¹  de la Rica, Roberto¹ 

[1] Hlth Res Inst Balearic Isl IdISBa, Multidisciplinary Sepsis Grp, Palma De Mallorca, Spain

[2] Son Llatzer Univ Hosp, Multidisciplinary Sepsis Unit, ICU, Palma De Mallorca, Spain

[3] Son Llatzer Univ Hosp, Emergency Dept, Palma De Mallorca, Spain

[4] Hlth Res Inst Balearic Isl IdISBa, Translat Res Aging & Longev TRIAL Grp, Palma De Mallorca, Spain

[5] Serv Salut Illes Balears, Serv Prevent Riesgos Laborales, Palma De Mallorca, Illes Balears, Spain

[6] Univ Balearic Isl, Chem Dept, Palma De Mallorca, Spain

关键词: SARS-CoV-2;    COVID-19;    Rapid diagnostic test;    Wearable;    Biosensor;    Smartphone;   

DOI  :  10.1016/j.snb.2021.130347

来源: Elsevier

【 摘 要 】

Detecting SARS-CoV-2 antigens in respiratory tract samples has become a widespread method for screening new SARS-CoV-2 infections. This requires a nasopharyngeal swab performed by a trained healthcare worker, which puts strain on saturated healthcare services. In this manuscript we describe a new approach for non-invasive COVID-19 diagnosis. It consists of using mobile biosensors for detecting viral antigens trapped in surgical face masks worn by patients. The biosensors are made of filter paper containing a nanoparticle reservoir. The nanoparticles transfer from the biosensor to the mask on contact, where they generate colorimetric signals that are quantified with a smartphone app. Sample collection requires wearing a surgical mask for 30 min, and the total assay time is shorter than 10 min. When tested in a cohort of 27 patients with mild or no symptoms, an area under the receiving operating curve (AUROC) of 0.99 was obtained (96.2 % sensitivity and 100 % specificity). Serial measurements revealed a high sensitivity and specificity when masks were worn up to 6 days after diagnosis. Surgical face masks are inexpensive and widely available, which makes this approach easy to implement anywhere. The excellent sensitivity, even when tested with asymptomatic patient samples, along with the mobile detection scheme and non-invasive sampling procedure, makes this biosensor design ideal for mass screening.

【 授权许可】

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