A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging

SENSORS AND ACTUATORS B-CHEMICAL

卷:192

Article

Bell, Laurence¹  Seshia, Ashwin¹  Lando, David²  Laue, Ernest²  Palayret, Matthieu³  Lee, Steven F.³  Klenerman, David³ 

[1] Cambridge Nanosci Ctr, Cambridge CB3 0FF, England

[2] Dept Biochem, Cambridge CB2 1GA, England

[3] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

关键词: Microfluidics;    Single cell analysis;    Hydrodynamic trapping;    PALM imaging;    Single molecule localisation microscopy;   

DOI  :  10.1016/j.snb.2013.10.002

来源: Elsevier

【 摘 要 】

We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.

【 授权许可】

Free