Multi-wavelength fluorescence detection of submicromolar concentrations using a filter-free fluorescence sensor

SENSORS AND ACTUATORS B-CHEMICAL

卷:256

Article

Choi, Yong Joon¹  Takahashi, Kazuhiro¹  Misawa, Nobuo¹  Hizawa, Takeshi¹  Iwata, Tatsuya¹  Sawada, Kazuaki¹ 

[1] Toyohashi Univ Technol, Dept Elect & Elect Informat Engn, Toyohashi, Aichi 4418580, Japan

关键词: Filter-free;    Fluorescence sensor;    Biosensor;    Multi-wavelength detection;    FITC;    Texas red;   

DOI  :  10.1016/j.snb.2017.09.077

来源: Elsevier

【 摘 要 】

In this paper, we report on the development of a filter-free fluorescence sensor for the detection of multiple fluorescence wavelengths from a solution of fluorescent dyes with submicromolar concentrations. The filter-free fluorescence sensor has a photo-gate structure, and the intensities of excitation light and fluorescence can be calculated from the photocurrents for the different gate voltages. This sensor makes it possible to measure various wavelengths without the need for optical filters or mirrors. The separation ability of excitation light and fluorescence in the developed sensor was more than 1000:1 for an LED light source. Fluorescence measurements were carried out for fluorescein isothiocyanate isomer (FITC) and sulforhodamine 101 acid chloride (Texas Red) dyes. Based on our calculations, the excitation light and fluorescence from submicromolar concentrations of FITC, Texas Red, and a mixed solution were successfully separated and simultaneously detected. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

Free