An ultra low-cost smartphone device for in-situ monitoring of acute organophosphorus poisoning for agricultural workers

SENSORS AND ACTUATORS B-CHEMICAL

卷:275

Article

Chang, Yu-Chung¹  Ge, Xiaoxiao¹  Wang, Li-Ju¹  Lee, Stephen Sauchi²  Paulsen, Michael H.³  Khan, Qaiser M.⁴  Khalid, Zafar M.⁴  Bhalli, Javed A.⁴  Waheed, Usman⁴  Simpson, Christopher D.³  Du, Dan¹  Li, Lei¹  Lin, Yuehe¹ 

[1] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA

[2] Univ Idaho, Dept Stat Sci, Moscow, ID 83844 USA

[3] Univ Washington, Dept Environm & Occupat Hlth Sci, Seattle, WA 98195 USA

[4] Natl Inst Biotechnol & Genet Engn, POB 577,Jhang Rd, Faisalabad, Pakistan

关键词: Organophosphorus poisoning;    Point-of-care diagnostics;    Mobile device;   

DOI  :  10.1016/j.snb.2018.08.009

来源: Elsevier

【 摘 要 】

In this work, we present an ultra-low-cost smartphone device for in situ quantification of OP poisoning severity. The performance of the lens-less smartphone spectrum apparatus (LeSSA) is evaluated using standard human Interleukin-6 (IL-6) immunoassay kits. Upon dose-response curve fitting, LeSSA demonstrates an accuracy of 99.5%. The limit of detection (LOD) of LeSSA was evaluated through comparison of 6.4 pg/ml with standard laboratory grade UV-vis spectrophotometer at 5.5 pg/ml. Evaluating the capacity of LeSSA in spike solution by combining plasma cholinesterase (PChE) and human plasma shows consistency at agreement of 97.6% between LeSSA and the laboratory instrument. For application demonstration, the activity of PChE for 24 agricultural workers' plasma samples was measured with LeSSA, showing exceptional agreement (r2 = 0.92) with the laboratory instrument reference. In addition to near laboratory grade accuracy, the total manufacturing cost of LeSSA is only $20 USD highlighting it's affordability. With LeSSA, clinicians can evaluate OP poisoning severity without the need to transport patient samples to facilities at far distances. Utilizing LeSSA, immediate results can be used for administration of appropriate treatment.

【 授权许可】

Free