High-performance electrochemical immunomagnetic assay for breast cancer analysis

SENSORS AND ACTUATORS B-CHEMICAL

卷:308

Article

Freitas, Maria¹  Nouws, Henri P. A.¹  Keating, Elisa^2,3  Delerue-Matos, Cristina¹ 

[1] Politecn Porto, Inst Super Engn Porto, REQUIMTE LAQV, Rua Dr Antonio Bernardino de Almeida 431, P-4200072 Porto, Portugal

[2] Univ Porto, Fac Med, Dept Biomed, Unit Biochem, Al Prof Hemani Monteiro, P-4200319 Porto, Portugal

[3] CINTESIS Ctr Hlth Technol & Serv Res, Rua Dr Placido da Costa, P-4200450 Porto, Portugal

关键词: Breast cancer;    HER2-ECD;    SK-BR-3;    Electrochemical immunoassay;    Magnetic beads;    Screen printed electrodes;   

DOI  :  10.1016/j.snb.2020.127667

来源: Elsevier

【 摘 要 】

Despite the evolution of targeted therapies in oncology, some challenges such as screening and early diagnosis of cancer-related biomarkers still remain. The analysis of the Human Epidermal growth factor Receptor 2 (HER2) in biological fluids provides essential information for effective treatments. In this work we report the development of an electrochemical immunomagnetic bioassay for the analysis of the extracellular domain of HER2 (HER2-ECD) in human serum and cancer cells. Biomodified carboxylic acid functionalized magnetic beads (COOH-MBs) were used as the capture probe and an antibody labelled with alkaline phosphatase (AP) as the signalling probe. In the presence of HER2-ECD a sandwich complex was formed on the MBs, which were magnetically attracted to the surface of a screen-printed carbon electrode (SPCE). After the addition of 3-indoxyl phosphate and silver ions, used as the enzymatic substrate, the immunological interaction was detected by linear sweep voltammetry. Two linear concentration ranges were established: one between 5.0 and 50 ng/mL and another between 50 and 100 ng/mL. The developed assay provided a clinically useful detection limit (2.8 ng/mL) and has an adequate precision (V-x0 < 5%). The assay provided accurate results and was selective towards the target biomarker. Additionally, CTCs were analysed in human serum and a detection limit of 3 cells/mL was achieved for the HER + breast cancer cell line SK-BR-3.

【 授权许可】

Free