On-chip real-time monitoring of multiple displacement amplification of DNA

SENSORS AND ACTUATORS B-CHEMICAL

卷:293

Article

Bruijns, B. B.^1,2  Costantini, F.^3,4  Lovecchio, N.⁵  Tiggelaar, R. M.^1,6  Di Timoteo, G.⁷  Nascetti, A.³  de Cesare, G.⁵  Gardeniers, J. G. E.¹  Caputo, D.⁵ 

[1] Univ Twente, MESA Inst Nanotechnol, Mesoscale Chem Syst, Drienerlolaan 5, NL-7522 NB Enschede, Netherlands

[2] Saxion Univ Appl Sci, Life Sci Engn & Design, MH Tromplaan 28, NL-7513 AB Enschede, Netherlands

[3] Sapienza Univ Rome, Sch Aerosp Engn, Via Salaria 851-881, I-00138 Rome, Italy

[4] Sapienza Univ Rome, Dept Chem, Ple Aldo Moro 5, I-00186 Rome, Italy

[5] Sapienza Univ Rome, Dept Informat Engn Elect & Telecommun, Via Eudossiana 18, I-00184 Rome, Italy

[6] Univ Twente, MESA Inst Nanotechnol, NanoLab Cleanroom, Drienerlolaan 5, NL-7522 NB Enschede, Netherlands

[7] Sapienza Univ Rome, Dept Biol & Biotechnol, Ple Aldo Moro 5, I-00186 Rome, Italy

关键词: MDA;    DNA amplification;    Real-time monitoring;    Fluorescence;    Lab-on-a-chip;   

DOI  :  10.1016/j.snb.2019.04.144

来源: Elsevier

【 摘 要 】

The integration of systems for real-time monitoring of DNA amplification reactions is rather limited, because the heating system, the temperature control and the detection system are usually realized with separate modules. In this work, a lab-on-a-chip system for real-time monitoring of the multiple displacement amplification reaction (MDA) is presented. The amplification and detection unit consists of a system-on-glass (SoG) coupled to a microfluidic chip. The SoG includes thin film metallic resistors and amorphous silicon temperature sensors to control the temperature, as well as amorphous silicon photosensors and an interference filter for fluorescence detection on a single glass plate. The amplification reaction is carried out in the microfluidic chip made of cyclic olefin copolymer (COC). By using this setup, the multiple displacement amplification reaction can be monitored in real-time using the fluorophore [Ru(phen)(2)(dPPz)](2+).

【 授权许可】

Free