Real-time recognition of Mycobacterium tuberculosis and lipoarabinomannan using the quartz crystal microbalance

SENSORS AND ACTUATORS B-CHEMICAL

卷:174

Article

Hiatt, Leslie A.¹  Cliffel, David E.¹ 

[1] Vanderbilt Univ, Dept Chem, Stevenson Ctr 7330, VU Stn B 351822, Nashville, TN 37235 USA

关键词: Quartz crystal microbalance;    Lipoarabinomannan;    Mycobacterium tuberculosis;    Immunosensor;    Langmuir isotherm;    Equilibrium association constant;    Steric hindrance effects;    Kinetic analysis;    Antibody adsorption;   

DOI  :  10.1016/j.snb.2012.06.095

来源: Elsevier

【 摘 要 】

A quartz crystal microbalance (QCM) immunosensor has been successfully employed to screen for both whole Mycobacteria tuberculosis (Mtb) bacilli and a Mtb surface antigen. lipoarabinomannan (LAM). One of the most abundant components of the Mtb cell surface. LAM, may be detected without the presence of the entire bacterium. Using available antibodies with proven utility in enzyme-linked immunoassays (ELISAs), a sensor was designed to measure Mtb bacilli and LAM. Equilibrium association constants (K-a) were determined for the interaction of Mtb with immobilized alpha-LAM and anti-H37Rv antibodies, where avidity was seen to strengthen this interaction and provide for greater binding than might have otherwise been achieved. The binding of LAM to immobilized alpha-LAM had a high associate rate constant (k(a)) allowing for rapid detection. Evaluating these binding constants helped to compare the sensitivity of these immunosensors to conventional ELISAs. The use of these assays with better antibodies may allow for immunosensor use in determining LAM as a point-of-care (POC) diagnostic for Mtb. (C) 2012 Elsevier B.V. All rights reserved.

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