Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

SENSORS AND ACTUATORS B-CHEMICAL

卷:263

Article

Al Rubaye, Ali¹  Nabok, Alexei¹  Catanante, Gaelle²  Marty, Jean-Louis²  Takacs, Ezster³  Szekacs, Andras³ 

[1] Sheffield Hallam Univ, Mat & Engn Res Inst, Sheffield, S Yorkshire, England

[2] Univ Perpignan, Dept Biochem & Mol Biol, Perpignan, France

[3] NARIC, Agroenvironm Res Inst, Budapest, Hungary

关键词: Aptamer;    Ochratoxin A;    Label-free optical biosensor;    Total internal reflection ellipsometry;    Binding kinetics;   

DOI  :  10.1016/j.snb.2018.01.220

来源: Elsevier

【 摘 要 】

The current work is a continuation of our research targeted the development of novel optical sensing technologies for detection of mycotoxins. The method of (TIRE) was developed in the last decade as a combination of spectroscopic ellisometry and SPR and was proved to be a highly sensitive analytical tool in bio-sensing particularly attractive for detection of low molecular weight analytes, such as mycotoxins. The use of aptamers as highly specific artificial molecular receptors to ochratoxin A (OTA) in conjunction with the method Total Internal Reflection Ellipsometry (TIRE) is reported here for the first time. Our results showed a possibility of label -free optical detection of OTA down to 0.01 ppb in concentration in direct assay with specific aptamer. The kinetics of aptamer/OTA binding was studied with dynamic TIRE spectral measurements and allowed evaluating the affinity constant KD =1.810(-8) Mol which is characteristic for highly specific aptamer/OTA binding. (C) 2018 Published by Elsevier B.V.

【 授权许可】

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