Electrochromic biosensors based on screen-printed Prussian Blue electrodes

SENSORS AND ACTUATORS B-CHEMICAL

卷:290

Article

Aller-Pellitero, Miguel¹  Fremeau, Josephine²  Villa, Rosa^1,3  Guirado, Gonzalo⁴  Lakard, Boris²  Hihn, Jean-Yves²  Javier del Campo, F.¹ 

[1] Esfera UAB, CSIC, IMB, CNM, Campus Univ Autonoma Barcelona, Barcelona 08193, Spain

[2] Univ Bourgogne Franche Comte, CNRS, UMR 6214, Inst UTINAM, 16 Route Gray, F-25030 Besancon, France

[3] CIBER BBN, Networking Ctr Bioengn Biomat & Nanomed, Barcelona, Spain

[4] Univ Autonoma Barcelona, Dept Quim, Fac Ciencies, Cerdanyola Del Valles 08193, Spain

关键词: Prussian Blue electrodes;    Screen-printed electrodes;    Spectroelectrochemistry;    Biosensors;    Hydrogen peroxide detection;    Glucose sensing;   

DOI  :  10.1016/j.snb.2019.03.100

来源: Elsevier

【 摘 要 】

Prussian Blue (PB)-modified graphite screen-printed electrodes are increasingly being used in electrochemical biosensors. However, they do not allow the observation of the electrochromism of PB. This work presents the construction of PB-based, electrochromic screen-printed biosensors. Although electrically more resistive than their graphite counterparts, these new PB-based electrodes enable both the amperometric and colorimetric detection of hydrogen peroxide. This is the first time that this has been achieved using screen-printed electrodes, and we demonstrate it spectroelectrochemically on a glucose biosensor. The biosensor electrochemical performance equals that of previously reported PB/graphite electrodes, being able to detect down to 4 mu M H2O2 and 54 mu M glucose. At the same time, and in contrast to PB/graphite electrodes, the new PB-based electrodes afford the optical detection of these two analytes down to 1.2 mu M and 15 mu M, respectively. The dynamic ranges of the glucose biosensors obtained at the PB-based electrodes are 0.1-1 mM (amperometric) and 0.025-2.5 mM (Colorimetric), matching the physiological glucose concentration range in body fluids other than blood or serum.

【 授权许可】

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