Protein imprinted materials designed with charged binding sites on screen-printed electrode for microseminoprotein-beta determination in biological samples

SENSORS AND ACTUATORS B-CHEMICAL

卷:223

Article

Rebelo, Tania S. C. R.^1,2,3  Pereira, Carlos M.⁴  Sales, M. Goreti F.¹  Noronha, J. P.²  Silva, Fernando⁴ 

[1] Inst Politecn Porto, Inst Super Engn, BioMark CINTESIS ISEP, Oporto, Portugal

[2] Univ Nova Lisboa, Fac Ciencias & Tecnol, Dept Quim, LAQV,REQUIMTE, Caparica, Portugal

[3] Univ Porto, Fac Med Dent, Lab Farmacol & Biocompatibilidade Celular, P-4100 Oporto, Portugal

[4] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, Ctr Invest Quim Linha 4, P-4100 Oporto, Portugal

关键词: Microseminoprotein-beta;    Caffeic acid;    Electrochemical biosensor;    Protein imprinted materials;    Screen printed electrodes;    Urine;    Serum;   

DOI  :  10.1016/j.snb.2015.09.133

来源: Elsevier

【 摘 要 】

In the past few years a large effort is being made aiming at the development of fast and reliable tests for cancer biomarkers. Protein imprinted sensors can be a fast and reliable strategy to develop tailor made sensors for a large number of relevant molecules. This work aims to produce, optimize and use in biological samples a biosensor for microseminoproteinbeta (MSMB). Caffeic acid (CAF) electropolimerization was performed in the presence of microseminoprotein-beta (MSMB) creating target protein specific cavities on the surface of a screen-printed carbon. Dopamine was introduced as charged monomer labelling the binding site and was allowed to self-organize around the protein. The subsequent electropolimerization was made by applying a constant potential of +2.0 V, for 30s, on a carbon screen-printed electrode, immersed in a solution of protein and CAF prepared in phosphate buffer. The sensor with charged monomers showed a more sensitive response, with an average slope of -7.59 mu A/decade, linear concentration range of 0.5-100 ng/mL and a detection limit of 0.12 ng/mL. The corresponding non-imprinted sensor displayed an inconsistent response over the range of the calibration curve. The biosensor was successfully applied to the analysis of MSMB in serum and urine samples. (C) 2015 Elsevier B.V. All rights reserved.

【 授权许可】

Free