Focusing-free impedimetric differentiation of red blood cells and leukemia cells: A system optimization

SENSORS AND ACTUATORS B-CHEMICAL

卷:307

Article

Bilican, Ismail¹  Guler, Mustafa Tahsin²  Serhatlioglu, Murat³  Kirindi, Talip⁴  Elbuken, Caglar³ 

[1] Aksaray Univ, Sci & Technol Applicat & Res Ctr, TR-68100 Aksaray, Turkey

[2] Kirikkale Univ, Dept Phys, TR-71450 Kirikkale, Turkey

[3] Bilkent Univ, Inst Mat Sci & Nanotechnol, UNAM Natl Nanotechnol Res Ctr, TR-06800 Ankara, Turkey

[4] Kirikkale Univ, Fac Educ, Dept Math & Sci Educ, TR-71450 Kirikkale, Turkey

关键词: Impedance spectroscopy;    Red blood cell;    White blood cell;    Leukemia;    Microfluidic flow cytometry;   

DOI  :  10.1016/j.snb.2019.127531

来源: Elsevier

【 摘 要 】

A focusing-free microfluidic impedimetric cell detection system is developed. The effect of the channel dimensions, solution conductivity, excitation voltage, and particle size on impedimetric signal outputs were optimized to increase the sensitivity of the system. Conventional microfabrication techniques were adapted to obtain low height, resealable microchannels. The geometry optimization was performed by a combination of analytical, numerical and experimental approaches. The results demonstrate that reliable impedimetric particle differentiation can be achieved without any labeling or particle focusing. The system parameters were studied and rule-of-thumb design criteria were provided. Finally, using the developed system, red blood cells and leukemia cells were experimentally detected and differentiated. Thanks to its simplicity, the focusing-free cell differentiation system may find applications in several cellular diagnostic uses.

【 授权许可】

Free