Label-free optical monitoring of proteolytic reaction products using nanoporous silica colloidal assembly

SENSORS AND ACTUATORS B-CHEMICAL

卷:262

Article

Shtenberg, Giorgi¹  Massad-Ivanir, Naama²  Khabibullin, Amir³  Zharov, Ilya³  Segal, Ester^2,4 

[1] Volcani Ctr, ARO, Inst Agr Engn, IL-50250 Bet Dagan, Israel

[2] Technion Israel Inst Technol, Dept Biotechnol & Food Engn, IL-3200003 Haifa, Israel

[3] Univ Utah, Dept Chem, Salt Lake City, UT 84112 USA

[4] Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, IL-3200003 Haifa, Israel

关键词: Biosensors;    Colloidal assembly;    Enzyme;    Protease;    Mass spectrometry;    Silica;    Interferometer;   

DOI  :  10.1016/j.snb.2018.02.057

来源: Elsevier

【 摘 要 】

An optical biosensor for rapid monitoring of proteolytic activity is constructed by immobilization of pro teases onto multifunctional silica colloidal assembly (SCA). The SCA serves as Fabry-Perot thin film, which is highly sensitive to the presence of biomolecules (e.g., enzymes, proteins and short peptides) within the nanostructure. Moreover, the SCA acts as a size-exclusion element, allowing to entrap and to concentrate the enzymatic degradation products for downstream mass spectrometry analysis for substrate profiling and cleavage sites identification. (C) 2018 Elsevier B.V. All rights reserved.

【 授权许可】

Free