Effect of handling on neurotransmitter profile in pig brain according to fear related behaviour

PHYSIOLOGY & BEHAVIOR

卷:167

Article

Arroyo, Laura¹  Carreras, Ricard²  Valent, Daniel¹  Pena, Raquel^1,3  Mainau, Eva⁴  Velarde, Antonio²  Sabria, Josefa⁵  Bassols, Anna^1,3 

[1] Univ Autonoma Barcelona, Fac Vet, Dept Bioquim & Biol Mol, Cerdanyola Del Valles 08193, Spain

[2] IRTA, Anim Welf Subprogram, Veinat Sies S-N, Monells 17121, Spain

[3] Univ Autonoma Barcelona, Fac Vet, Serv Bioquim Clin Vet, Cerdanyola Del Valles 08193, Spain

[4] Univ Autonoma Barcelona, Fac Vet, Dept Ciencia Anim & Aliments, Cerdanyola Del Valles 08193, Spain

[5] Univ Autonoma Barcelona, Inst Neurociencies, Fac Med, Dept Bioquim & Biol Mol, Cerdanyola Del Valles 08193, Spain

关键词: Brain;    Dopamine;    Pig;    Serotonin;    Stress;    Tonic immobility;   

DOI  :  10.1016/j.physbeh.2016.10.005

来源: Elsevier

【 摘 要 】

Chemical neurotransmitters (NT) are principal actors in all neuronal networks of animals. The central nervous system plays an important role in stress susceptibility and organizes the response to a stressful situation through the interaction of the dopaminergic and the serotonergic pathways, leading to the activation of the hypothalamus-pituitary-adrenal axis (HPA). This study was designed to investigate: a) the effects of stressful handling of pigs at the slaughterhouse on the neurotransmitter profile in four brain areas: amygdala, prefrontal cortex (PFC), hippocampus and hypothalamus, and b) whether the alterations in the brain NT profile after stressful handling were associated with fear, determined by the tonic immobility (TI) test In the first place, the characterization of the NT profile allowed to distinguish the four brain areas in a principal component analysis. The most crucial pathway involved in the reaction of pigs to a stressful handling was the serotonergic system, and changes were observed in the amygdala with a decrease in serotonin (5-HT) and total indoleamines, and in the hippocampus, where this pathway was activated. Fearful and non-fearful pigs did not show significant differences in their NT profile in control conditions, but when subjected to a stressful handling in the slaughterhouse, fearful animals showed a significant variation in the serotonin pathway and, in a lesser extent, the dopamine (DA) pathway. In conclusion, the existence of an underlying biological trait - possibly fearfulness - may be involved in the pig's response toward stressful challenges, and the serotonergic system seems to play a central role in this response. (C) 2016 Elsevier Inc All rights reserved.

【 授权许可】

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