Modulation of glutamate levels and Na+,K+-ATPase activity contributes to the chrysin memory recovery in hypothyroidism mice

PHYSIOLOGY & BEHAVIOR

卷:222

Article

Bortolotto, Vandreza Cardoso¹  Araujo, Stifani Machado¹  Pinheiro, Franciane Cabral¹  Poetini, Marcia Rosula¹  de Paula, Mariane Trindade¹  Meichtry, Luana Barreto¹  de Almeida, Francielli Polet¹  Santos Musachio, Elize Aparecida¹  Guerra, Gustavo Petri¹  Prigol, Marina¹ 

[1] Univ Fed Pampa, Lab Avaliacoes Farmacol & Toxicol Aplicadas Mol B, Campus Itaqui, BR-97650000 Itaqui, RS, Brazil

关键词: Flavonoid;    Chrysin;    Hypothyroidism;    Methimazole;    Glutamate;    Na+;    K+-ATPase;    Memory deficit;   

DOI  :  10.1016/j.physbeh.2020.112892

来源: Elsevier

【 摘 要 】

Abnormalities in the thyroid hormones, like in hypothyroidism, are closely related to dementia and Alzheimer's disease demonstrating the main symptom of these disorders: memory deficit. In this study we evaluated the effect of chrysin on deficit spatial and aversive memories and the contribution of glutamatergic, cholinergic pathways and Na+, K+-ATPase activity on hippocampus and prefrontal cortex in hypothyroid adult female mice C57BL/6. Hypothyroidism was induced by the continuous exposure to 0.1% methimazole (MTZ) in drinking water for 31 days. The exposure to MTZ was associated to low plasma levels of thyroid hormones (TH) compared to the control group on the 32nd. Subsequently, euthyroid and MTZ-induced hypothyroid mice received (intragastrically) either vehicle or chrysin (20 mg/kg) once a day for 28 consecutive days. After treatments mice performed the following behavioral assessments: open-field test (OFF), morris water maze (MWM) and passive avoidance test. Additionally, plasma TH levels were measured again, as well as glutamate levels, Na+,K+-ATPase and acetylcholinesterase (AChE) activities were analyzed in the hippocampus and prefrontal cortex of mice. Mice with hypothyroidism showed a deficit of spatial and aversive memory and chrysin treatment reversed these deficits. It also reduced the levels of glutamate and decreased Na tATPase activity in both cerebral structures in the hypothyroid mice compared with the euthyroid ones, with the exception of glutamate in the hippocampus, which was a partial reversal. AChE activity was not altered by treatments. Together, our results demonstrate that chrysin normalized hippocampal glutamate levels and Na+, K+-ATPase activity, which could be involved in the reversal of memory deficit.

【 授权许可】

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