Physiological responses during acute stress recovery depend on stress coping style in European sea bass, Dicentrarchus labrax

PHYSIOLOGY & BEHAVIOR

卷:216

Article

Ferrari, Sebastien^1,2  Rey, Sonia³  Hoglund, Erik⁴  Overli, Oyvind⁵  Chatain, Beatrice²  MacKenzie, Simon³  Begout, Marie-Laure¹ 

[1] IFREMER, Fisheries Res Lab, F-17137 Lhoumeau, France

[2] Univ Montpellier, MARBEC, CNRS, IFREMER,IRD, Palavas Les Flots, France

[3] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland

[4] Norwegian Inst Water Res NIVA, N-0349 Oslo, Norway

[5] Norwegian Univ Life Sci, Fac Vet Med, Dept Food Safety & Infect Biol, N-0033 Oslo, Norway

关键词: Fish;    Behavior;    Physiology;    Personality;    Brain;    Transcriptomic;   

DOI  :  10.1016/j.physbeh.2020.112801

来源: Elsevier

【 摘 要 】

Individual stress coping style (reactive, intermediate and proactive) was determined in 3 groups of 120 pit tagged European seabass using the hypoxia avoidance test. The same three groups (no change in social composition) were then reared according to the standards recommended for this species. Then, 127 days later, individuals initially characterized as reactive, intermediate or proactive were submitted to an acute confinement stress for 30 min. Blood samples were taken to measure plasma cortisol levels 30 min (Stress30) or 150 min (Stress150) after the end of the confinement stress. Individuals were then sacrificed to sample the telencephalon in order to measure the main monoamines and their catabolites (at Stress30 only). Individuals from Stress150 were sampled for whole brain for a transcriptomic analysis. The main results showed that reactive individuals had a lower body mass than intermediate individuals which did not differ from proactive individuals. The physiological cortisol response did not differ between coping style at Stress30 but at Stress150 when intermediate and proactive individuals had recovered pre stress levels, reactive individuals showed a significant higher level illustrating a modulation of stress recovery by coping style. Serotonin turnover ratio was higher in proactive and reactive individuals compared to intermediate individuals and a significant positive correlation was observed with cortisol levels whatever the coping style. Further, the confinement stress led to a general increase in the serotonin turnover comparable between coping styles. Stress150 had a significant effect on target mRNA copy number (Gapdh mRNA copy number decreased while ifrd1 mRNA copy number increased) and such changes tended to depend upon coping style.

【 授权许可】

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