Fluorescence correlation spectroscopy of CdSe/ZnS quantum dot optical bioimaging probes with ultra-thin biocompatible coatings

OPTICS COMMUNICATIONS

卷:281

Article

Murcia, Michael J.¹  Shaw, David L.¹  Long, Eric C.¹  Naumann, Christoph A.¹ 

[1] Indiana Univ Purdue Univ, Dept Chem & Chem Biol, Purdue Sch Sci, Indianapolis, IN 46202 USA

关键词: CdSe/ZnS quantum dots;    FCS;   

DOI  :  10.1016/j.optcom.2007.07.069

来源: Elsevier

【 摘 要 】

The colloidal stabilities and emission properties of CdSe/ZnS quantum dot (QD) optical probes capped with a variety of thin, hydrophilic surface coatings were studied using confocal fluorescence correlation spectroscopy. These coatings are based on mercaptoethanol, mercaptopropionic acid (with and without conjugated aminoethoxyethanol), lipopolymers (DSPE-PEG2000), cysteine (Cys), and a variety of Xaa-Cys dipeptides. The study shows that QDs with thin hydrophilic coatings can be designed that combine good colloidal stability and excellent emission properties (brightness). Furthermore, there is a general correlation between colloidal stability and brightness. The experiments reported illustrate that QDs with multiple types of thin coatings can be created for optical imaging applications in a biological environment while also maintaining a size below 10 nm. (C) 2007 Elsevier B.V. All rights reserved.

【 授权许可】

Free