Evaluation of a eukaryote phylogenetic microarray for environmental monitoring of marine sediments

MARINE POLLUTION BULLETIN

卷:154

Article

Lekang, Katrine^1,2  Lanzedn, Anders^3,4  Jonassen, Inge⁵  Thompson, Eric^1,6,7  Troedsson, Christofer^1,7,8 

[1] Univ Bergen, Dept Biol, Bergen, Norway

[2] Univ Oslo, Dept Pharm, Oslo, Norway

[3] AZTI Tecnalia, Marine Res Div, Pasaia, Spain

[4] Ikerbasque, Basque Fdn Sci, Bilbao 48011, Spain

[5] Univ Bergen, Dept Informat, Computat Biol Unit, Bergen, Norway

[6] Univ Bergen, Sars Int Ctr Marine Mol Biol, Bergen, Norway

[7] NORCE, Bergen, Norway

[8] Ocean Bergen AS, Bergen, Norway

关键词: Biodiversity;    Monitoring;    Sediment;    Microarray;    Microorganisms;    Ecosystem;   

DOI  :  10.1016/j.marpolbul.2020.111102

来源: Elsevier

【 摘 要 】

Increased exploitation of resources in sensitive marine ecosystems emphasizes the importance of knowledge regarding ecological impacts. However, current bio-monitoring practices are limited in terms of target-organisms and temporal resolution. Hence, developing new technologies is vital for enhanced ecosystem understanding. In this study, we have applied a prototype version of a phylogenetic microarray to assess the eukaryote community structures of marine sediments from an area with ongoing oil and gas drilling activity. The results were compared with data from both sequencing (metabarcoding) and morphology-based monitoring to evaluate whether microarrays were capable of detecting ecosystem disturbances. A significant correlation between microarray data and chemical pollution indicators, as well as sequencing-based results, was demonstrated, and several potential indicator organisms for pollution-associated parameters were identified, among them a large fraction of microorganisms not covered by traditional morphology-based monitoring. This suggests that microarrays have a potential in future environmental monitoring.

【 授权许可】

Free