16S rRNA Gene Survey of Microbial Communities in Winogradsky Columns

PLoS One

Ethan A. Rundell¹  David J. Esteban¹  Lois M. Banta²  Corey D. Watts²  Doyle V. Ward³  Bruce Birren³ 

[1] Department of Biology, Vassar College, Poughkeepsie, New York, United States of America;Department of Biology, Williams College, Williamstown, Massachusetts, United States of America;Genome Sequencing Center, Broad Institute, Cambridge, Massachusetts, United States of America

关键词: Ponds;    Sediment;    Biomarkers;    Sulfur;    Community structure;    Shannon index;    Sulfates;    Biodiversity;   

DOI  :  10.1371/journal.pone.0104134

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

A Winogradsky column is a clear glass or plastic column filled with enriched sediment. Over time, microbial communities in the sediment grow in a stratified ecosystem with an oxic top layer and anoxic sub-surface layers. Winogradsky columns have been used extensively to demonstrate microbial nutrient cycling and metabolic diversity in undergraduate microbiology labs. In this study, we used high-throughput 16s rRNA gene sequencing to investigate the microbial diversity of Winogradsky columns. Specifically, we tested the impact of sediment source, supplemental cellulose source, and depth within the column, on microbial community structure. We found that the Winogradsky columns were highly diverse communities but are dominated by three phyla: Proteobacteria, Bacteroidetes, and Firmicutes. The community is structured by a founding population dependent on the source of sediment used to prepare the columns and is differentiated by depth within the column. Numerous biomarkers were identified distinguishing sample depth, including Cyanobacteria, Alphaproteobacteria, and Betaproteobacteria as biomarkers of the soil-water interface, and Clostridia as a biomarker of the deepest depth. Supplemental cellulose source impacted community structure but less strongly than depth and sediment source. In columns dominated by Firmicutes, the family Peptococcaceae was the most abundant sulfate reducer, while in columns abundant in Proteobacteria, several Deltaproteobacteria families, including Desulfobacteraceae, were found, showing that different taxonomic groups carry out sulfur cycling in different columns. This study brings this historical method for enrichment culture of chemolithotrophs and other soil bacteria into the modern era of microbiology and demonstrates the potential of the Winogradsky column as a model system for investigating the effect of environmental variables on soil microbial communities.

【 授权许可】

CC BY