Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

MARINE POLLUTION BULLETIN

卷:114

Article

Zhang, Ying¹  Wu, Huifeng²  Wei, Lei²  Xie, Zeping¹  Guan, Bo² 

[1] Binzhou Med Univ, Sch Basic Med Sci, 346,Guanhai Rd, Yantai 264003, Peoples R China

[2] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China

关键词: Metabolic profile;    Hypoxia;    Ruditapes philippinarum;    Proton nuclear magnetic resonance;    Metabolomics;   

DOI  :  10.1016/j.marpolbul.2016.08.066

来源: Elsevier

【 摘 要 】

Coastal hypoxia affects the survival, behavior, and reproduction of individual local marine organisms, and the abundance, biomass, and biodiversity of coastal ecosystems. In this study, we investigated the chronic effects of hypoxia on the metabolomics in the gills of Ruditapes (R.) philippinarum. The results indicated significant alterations in the metabolite profiles in the gills of the hypoxia-treated clams, in comparison with those maintained under normoxia. The levels of betaine, taurine, glycine, isoleucine, and alanine were significantly reduced, suggesting a disturbance of osmotic balance associated with hypoxia. Meanwhile, metabolites involved in energy metabolism, such as alanine and succinate, were also affected. Dramatic histopathological changes were observed in the gills and hepatopancreases of R. philippinarum grown in hypoxic waters, demonstrating tissue damages apparently caused by long-term exposure to hypoxia. Our findings suggest that hypoxia significantly affects the physiology of R. philippinarum, even at a sub-lethal level, and impedes health of the clams. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

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