Surveillance of human enteric viruses in coastal waters using concentration with methacrylate monolithic supports prior to detection by RT-qPCR

MARINE POLLUTION BULLETIN

卷:128

Article

Goncalves, Jose^1,2  Gutierrez-Aguirre, Ion²  Balasubramanian, Mukundh N.³  Zagorscak, Maja²  Ravnikar, Maja²  Turk, Valentina^1,4 

[1] Natl Inst Biol, Marine Biol Stn Piran, Fornace 41, SI-6330 Piran, Slovenia

[2] Natl Inst Biol, Dept Biotechnol & Syst Biol, Vecna Pot 111, SI-1000 Ljubljana, Slovenia

[3] BioSistemika LLC, Koprska 98, SI-1000 Ljubljana, Slovenia

[4] Univ Nova Gorica, Vipavska 15, SI-5000 Nova Gorica, Slovenia

关键词: Rotavirus;    Norovirus;    Faecal coliforms;    RT-qPCR;    Methacrylate monolithic columns;    Enteric virus concentration;   

DOI  :  10.1016/j.marpolbul.2018.01.040

来源: Elsevier

【 摘 要 】

This is the first surveillance study using methacrylate monolithic supports to concentrate environmental coastal water samples, prior to molecular target detection by RT-qPCR. Rotaviruses (RoV) and Noroviruses (NoV) were monitored in a polluted area at the Bay of Koper (Gulf of Trieste, Northern Adriatic Sea) and at a nearby bathing area and mussel farm areas. RoV and NoV are released into the Bay of Koper, with higher rates close to the discharge of the wastewater treatment plant, however, they can be detected at recreational and mussel farming areas. Our results showed that water bodies considered safe based on FC concentrations, can still have low, yet potentially infective, concentrations of human viruses.

【 授权许可】

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