Contrasting sex-dependent adaptations to synaptic physiology and membrane properties of prefrontal cortex interneuron subtypes in a mouse model of binge drinking

NEUROPHARMACOLOGY

卷:178

Article

Joffe, Max E.^1,2,3  Winder, Danny G.^1,3,4  Conn, P. Jeffrey^1,2,3 

[1] Vanderbilt Univ, Dept Pharmacol, Nashville, TN 37232 USA

[2] Warren Ctr Neurosci Drug Discovery, Nashville, TN 37232 USA

[3] Vanderbilt Ctr Addict Res, Nashville, TN 37232 USA

[4] Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA

关键词: Alcohol;    Prefrontal cortex;    Sex differences;    Synaptic physiology;    Parvalbumin;    Somatostatin;    Article type: research;   

DOI  :  10.1016/j.neuropharm.2020.108126

来源: Elsevier

【 摘 要 】

Alcohol use disorder (AUD) affects all sexes, however women who develop AUD may be particularly susceptible to cravings and other components of the disease. While many brain regions are involved in AUD etiology, proper prefrontal cortex (PFC) function is particularly important for top-down craving management and the moderation of drinking behaviors. Essential regulation of PFC output is provided by local inhibitory interneurons, yet how drinking affects interneuron physiology remains poorly understood, particularly in female individuals. To address this gap, we generated fluorescent reporter transgenic mice to label the two major classes of interneuron in deep layer prelimbic PFC, based on expression of parvalbumin (PV-IN) or somatostatin (SST-IN). We then interrogated PV-IN and SST-IN membrane and synaptic physiology in a rodent model of binge drinking. Beginning in late adolescence, mice received 3-4 weeks of intermittent access (IA) ethanol. We prepared acute brain slices one day after the last drinking session. PV-INs but not SST-INs from IA ethanol mice displayed increased excitability relative to controls, regardless of sex. On the contrary, synaptic adaptations to PV-INs differed based on sex. While drinking decreased excitatory synaptic strength onto PV-INs from female mice, PV-INs from IA ethanol male mice exhibited potentiated excitatory transmission relative to controls. In contrast, decreased synaptic strength onto SST-INs was observed following IA ethanol in all groups of mice. Together, these findings illustrate novel sex differences in drinking-related PFC pathophysiology. Discovering means to restore PV-IN and SST-IN dysfunction following extended drinking provides opportunities for developing new treatments for all AUD patients.

【 授权许可】

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