Alcohol drinking during early adolescence activates microglial cells and increases frontolimbic Interleukin-1 beta and Toll-like receptor 4 gene expression, with heightened sensitivity in male rats compared to females

NEUROPHARMACOLOGY

卷:197

Article

Silva-Gotay, Andrea¹  Davis, Jillian²  Tavares, Elizabeth R.²  Richardson, Heather N.^1,2 

[1] Univ Massachusetts, Neurosci & Behav Grad Program, Amherst, MA 01003 USA

[2] Univ Massachusetts, Dept Psychol & Brain Sci, Amherst, MA 01003 USA

关键词: Adolescence;    Alcohol;    Operant self-administration;    Binge drinking;    Prefrontal cortex;    mPFC;    Hippocampus;    Development;    Sex differences;    Male;    Female;    Rats;    Neuroinflammation;    Cytokines;    Iba1;    Interleukin-1 beta;    Il1b;    Toll-like receptor 4;    Tlr4;    Microglia;   

DOI  :  10.1016/j.neuropharm.2021.108698

来源: Elsevier

【 摘 要 】

Adolescent drinking is risky because neural circuits in the frontal lobes are undergoing maturational processes important for cognitive function and behavioral control in adulthood. Previous studies have shown that myelinated axons in the medial prefrontal cortex (mPFC) are particularly sensitive to alcohol drinking, especially in males. Pro-inflammatory mediators like toll-like receptor 4 (TLR4) and interleukin-1 beta (IL1b) have been implicated in alcohol induced-inflammation and demyelination; thus, herein we test the hypothesis that voluntary alcohol drinking early in adolescence elicits a pro-inflammatory state that is more pronounced in the brain of males compared to females. Adolescent male and female Wistar rats self-administered sweetened alcohol or sweetened water from postnatal days 28-42 and separate sets of brains were processed for 1) immunolabeling for ionized calcium-binding adapter molecule 1 to analyze microglial cell morphology, or 2) qPCR analysis of gene expression of pro-inflammatory mediators. Binge drinking alcohol activated microglia in the mPFC and hippocampus of both males and females, suggesting that voluntary alcohol exposure initiates an inflammatory response. Il1b mRNA was upregulated in the mPFC of both sexes. Conversely, Tlr4 mRNA levels were elevated after drinking only in males, which could explain more robust effects of alcohol on myelin in this region in developing males compared to females. Il1b mRNA changes were not observed in the hippocampus, but alcohol elevated Tlr4 mRNA in both sexes, highlighting regional specificity in inflammatory responses to alcohol. Overall, these findings give insight into potential mechanisms by which low-to-moderate voluntary alcohol intake impacts the developing brain. This article is part of the special Issue on 'Vulnerabilities to Substance Abuse'.

【 授权许可】

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