Early-life stress exacerbates the effects of WIN55,212-2 and modulates the cannabinoid receptor type 1 expression

NEUROPHARMACOLOGY

卷:184

Article

Martin-Sanchez, Ana^1,2  Garcia-Baos, Alba¹  Castro-Zavala, Adriana¹  Alegre-Zurano, Laia¹  Valverde, Olga^1,2 

[1] Univ Pompeu Fabra, Dept Expt & Hlth Sci, Neurobiol Behav Res Grp GReNeC NeuroBio, Barcelona, Spain

[2] IMIM Hosp Mar Res Inst, Neurosci Res Program, Barcelona, Spain

关键词: Maternal separation;    WIN55, 212-2;    Cannabinoid receptor type 1;    Physical dependence;    Memory;    Iba1;   

DOI  :  10.1016/j.neuropharm.2020.108416

来源: Elsevier

【 摘 要 】

Early-life stress induces an abnormal brain development and increases the risk of psychiatric diseases, including depression, anxiety and substance use disorders. We have developed a reliable model for maternal neglect, named maternal separation with early weaning (MSEW) in CD1 mice. In the present study, we evaluated the long-term effects on anxiety-like behaviours, nociception as well as the Iba1-positive microglial cells in this model in comparison to standard nest (SN) mice. Moreover, we investigated whether MSEW alters the cannabinoid agonist WIN55,212-2 effects regarding reward, spatial and emotional memories, tolerance to different cannabinoid responses, and physical dependence. Adult male offspring of MSEW group showed impaired responses on spatial and emotional memories after a repeated WIN55,212-2 treatment. These behavioural impairments were associated with an increase in basolateral amygdala and hippocampal CB1-expressing fibres and higher number of CB1-containing cells in cerebellum. Additionally, MSEW promotes a higher number of Iba1-positive microglial cells in basolateral amygdala and cerebellum. As for the cannabinoid-induced effects, rearing conditions did not influence the rewarding effects of WIN55,212-2 in the conditioned place preference paradigm. However, MSEW mice showed a delay in the development of tolerance to the cannabinoid effects. Moreover, CB1-positive fibres were reduced in limbic areas in MSEW mice after cannabinoid withdrawal precipitated with the CB1 antagonist SR141617A. These findings support that early-life stress promotes behavioural and molecular changes in the sensitivity to cannabinoids, which are mediated by alterations in CB1 signalling in limbic areas and it induces an increased Iba1-microglial marker which could interfere in emotional memories formation.

【 授权许可】

Free