Adenylyl cyclases types 1 and 8 promote pro-survival pathways after ethanol exposure in the neonatal brain

NEUROBIOLOGY OF DISEASE

卷:33

Article

Conti, Alana C.¹  Young, Chainllie²  Olney, John W.²  Muglia, Louis J.¹ 

[1] Washington Univ, Dept Pediat, St Louis, MO 63110 USA

[2] Washington Univ, Dept Psychiat, St Louis, MO 63110 USA

关键词: Fetal alcohol syndrome;    Striatum;    Apoptosis;    Ethanol;    Phosphorylation;    Caspase-3;    Adenylyl cyclase;   

DOI  :  10.1016/j.nbd.2008.09.022

来源: Elsevier

【 摘 要 】

Although a wide range of developmental disabilities following fetal alcohol exposure are observed clinically, the molecular factors that determine the severity of these sequelae remain undefined. In mice exposed to ethanol, deletion of adenylyl cyclases (ACs) 1 and 8 exacerbates the neuroapoptosis that occurs in a prolonged post-treatment period; however, it remains unclear whether AC7 and AC8 are critical to the primary or secondary mechanisms underlying ethanol-induced neurodegeneration. Here we demonstrate that mice lacking AC7 and ACS (DKO) display significantly increased apoptosis in the striatum, a region sensitive to neuroapoptosis in the acute post-treatment period, compared to WT controls. The enhanced neuroapoptotic response observed in the striatum of DKO mice is accompanied by significant reductions in phosphorylation of known pro-survival proteins, insulin receptor substrate-1 (IRS-1), Akt and extracellular signal-regulated kinases (ERKs). These data suggest that AC7/AC8 are crucial activators of cell survival signaling pathways acutely following ethanol exposure and represent molecular factors that may directly modulate the severity of symptoms associated with Fetal Alcohol Syndrome. (c) 2008 Elsevier Inc. All rights reserved.

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