Differential effect of amyloid beta peptides on mitochondrial axonal trafficking depends on their state of aggregation and binding to the plasma membrane

NEUROBIOLOGY OF DISEASE

卷:114

Article

Zhang, Liang¹  Trushin, Sergey¹  Christensen, Trace A.²  Tripathi, Utkarsh¹  Hong, Courtney¹  Geroux, Rachel E.¹  Howell, Kyle G.^1,2  Poduslo, Joseph F.^1,3  Trushina, Eugenia^1,4 

[1] Mayo Clin, Dept Neurol, 200 First St SW, Rochester, MN 55905 USA

[2] Mayo Clin, Microscopy & Cell Anal Core Facil, 200 First St SW, Rochester, MN 55905 USA

[3] Mayo Clin, Dept Biochem & Mol Biol, 200 First St SW, Rochester, MN 55905 USA

[4] Mayo Clin, Dept Mol Pharmacol & Expt Therapeut, 200 First St SW, Rochester, MN 55905 USA

关键词: Alzheimer's disease;    Amyloid beta peptides;    Mitochondria;    Oligomers;    Atomic force microscopy;    Dynamic light scattering;    Fibrils;    Axonal transport;    Endocytosis;    Mitochondrial function;    Bioenergetics;    Electron microscopy;    Seahorse extracellular flux analyzer;    Mouse models of AD;    Primary neurons;    Plasma membrane binding;    Aggregation;   

DOI  :  10.1016/j.nbd.2018.02.003

来源: Elsevier

【 摘 要 】

Inhibition of mitochondrial axonal trafficking by amyloid beta (A beta) peptides has been implicated in early pathophysiology of Alzheimer's Disease (AD). Yet, it remains unclear whether the loss of motility inevitably induces the loss of mitochondrial function, and whether restoration of axonal trafficking represents a valid therapeutic target. Moreover, while some investigations identify A beta oligomers as the culprit of trafficking inhibition, others propose that fibrils play the detrimental role. We have examined the effect of a panel of A beta peptides with different mutations found in familial AD on mitochondrial motility in primary cortical mouse neurons. Peptides with higher propensity to aggregate inhibit mitochondrial trafficking to a greater extent with fibrils inducing the strongest inhibition. Binding of A beta peptides to the plasma membrane was sufficient to induce trafficking inhibition where peptides with reduced plasma membrane binding and internalization had lesser effect on mitochondrial motility. We also found that All peptide with Icelandic mutation A673T affects axonal trafficking of mitochondria but has very low rates of plasma membrane binding and internalization in neurons, which could explain its relatively low toxicity. Inhibition of mitochondrial dynamics caused by A beta peptides or fibrils did not instantly affect mitochondrial bioenergetic and function. Our results support a mechanism where inhibition of axonal trafficking is initiated at the plasma membrane by soluble low molecular weight A beta species and is exacerbated by fibrils. Since trafficking inhibition does not coincide with the loss of mitochondrial function, restoration of axonal transport could be beneficial at early stages of AD progression. However, strategies designed to block A beta aggregation or fibril formation alone without ensuring the efficient clearance of soluble A beta may not be sufficient to alleviate the trafficking phenotype.

【 授权许可】

Free