Spatial behavior and seizure-induced changes in c-fos mRNA expression in young and old rats

NEUROBIOLOGY OF AGING

卷:34

Article

Chawla, Monica K.^1,2  Penner, Marsha R.³  Olson, Kathy M.^1,2  Sutherland, Vicki L.⁴  Mittelman-Smith, Melinda A.⁵  Barnes, Carol A.^1,2 

[1] Univ Arizona, ARL Div Neural Syst Memory & Aging, Tucson, AZ 85724 USA

[2] Univ Arizona, Evelyn F McKnight Brain Inst, Tucson, AZ 85724 USA

[3] Univ Washington, Dept Psychol, Seattle, WA 98195 USA

[4] Bristol Myers Squibb Co, Dept Reprod Toxicol, New Brunswick, NJ USA

[5] Univ Arizona, Dept Pathol, Tucson, AZ 85724 USA

关键词: Aging;    Immediate early-genes;    Transcription;    Learning and memory;    Synaptic plasticity;    Seizures;   

DOI  :  10.1016/j.neurobiolaging.2012.10.017

来源: Elsevier

【 摘 要 】

The subcellular processes of gene induction and expression in the hippocampus are likely to underlie some of the known age-related impairments in spatial learning and memory. It is well established that immediate-early genes are rapidly and transiently induced in response to neuronal activity and this expression is required for stabilization of durable memories. To examine whether age-related memory impairment might be caused, in part, by differences in the level of cellular activation or subcellular processing, c-fos expression in CA1 pyramidal and dentate gyrus granule cells in the dorsal hippocampus of young and old rats was determined using fluorescence in situ hybridization and reverse transcription polymerase chain reaction. No significant age differences were found in the numbers of pyramidal or granule cells that show c-fos expression; however, c-fos mRNA transcripts were altered in these 2 cell types in aged animals. These findings suggest that though the networks of cells that participate in behavior or seizure-induced activity are largely maintained in aged rats, their RNA transcript levels are altered. This might, in part, contribute to cognitive deficits frequently observed with advancing age. (C) 2013 Elsevier Inc. All rights reserved.

【 授权许可】

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