Male and female factors that influence ICSI outcome in azoospermia or aspermia

REPRODUCTIVE BIOMEDICINE ONLINE

卷:18

Article; Proceedings Paper

Zorn, Branko¹  Virant-Klun, Irma²  Drobni, Saso¹  Sinkovec, Jasna³  Meden-Vrtovec, Helena² 

[1] Univ Med Ctr Ljubljana, Androl Ctr, Ljubljana, Slovenia

[2] Univ Med Ctr Ljubljana, Reprod Unit, Ljubljana, Slovenia

[3] Univ Med Ctr Ljubljana, Cytopath Unit, Dept Obstet & Gynecol, Ljubljana, Slovenia

关键词: aspermia;    azoospermia;    blastocyst;    female age;    ICSI;    sperm quality;   

DOI  :  10.1016/S1472-6483(10)60252-0

来源: Elsevier

【 摘 要 】

Factors that influenced the clinical results of 220 first-attempt intracytoplasmic sperm injection (ICSI) cycles with testicular spermatozoa were evaluated in 107 men with non-obstructive azoospermia, 72 with obstructive azoospermia and 41 with aspermia. Linear and logistic regression analysis showed that the fertilization rate depended positively on Johnsen score (P = 0.016) and on the type of ovarian stimulation: a higher fertilization rate was observed after ovarian stimulation with agonist and recombinant FSH than after stimulation with agonist and urinary menopausal gonadotrophin (P = 0.026). Embryo development to the blastocyst stage was predicted positively by the number of injected oocytes (P = 0.016) and negatively by male FSH concentration (P = 0.019). A higher proportion of blastocysts developed after the use of frozen-thawed spermatozoa in comparison to fresh spermatozoa (P = 0.034). Embryo development to the biastocyst stage influenced pregnancy (P = 0.002) and live birth outcomes (P = 0.005); live birth was also predicted by female age (P = 0.046). Embryo culture to day 5 in comparison to day 2 did not provide higher live birth rates. In azoospermia/aspermia, the ICSI outcome depends on both male factors (FSH, Johnsen score, sperm status and motility) and female factors (age, number of injected oocytes).

【 授权许可】

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