Increased incidence of mosaicism detected by FISH in murine blastocyst cultured in vitro

REPRODUCTIVE BIOMEDICINE ONLINE

卷:22

Article

Sabhnani, Tanya V.¹  Elaimi, Aisha²  Sultan, Hanan²  Alduraihem, Adel²  Serhal, Paul¹  Harper, Joyce C.^1,2 

[1] Univ Coll London Hosp, Ctr Reprod & Genet Hlth, London, England

[2] UCL, Inst Womens Hlth, Ctr PG & D, London, England

关键词: FISH;    in-vitro culture;    morphology;    mosaicism;    murine embryos;   

DOI  :  10.1016/j.rbmo.2011.01.011

来源: Elsevier

【 摘 要 】

The majority of in-vitro-derived human preimplantation embryos are chromosomally abnormal but whether the same pattern exists in vivo is unknown. This would be impossible to demonstrate in humans. Hence we chose murine embryos to study this difference owing to their ease of manipulation and compared the incidence of mosaicism between in-vivo- and in-vitro-cultured embryos. Two groups of embryos were analysed. Group A (in vitro) were obtained 48 h following superovulation and cultured in vitro until the blastocyst stage. Fluorescent in-situ hybridization (FISH) was performed at different stages that included the cleavage, morula and blastocyst stage. Group B (in vivo) were obtained on day 2 or day 5 and FISH was performed immediately without culture. There was an increase in chromosomal mosaicism seen from the cleavage stage up to the blastocyst stage in the in-vitro culture group. Overall chromosomal abnormality from day 3 to day 5 was found to be 30% (28/94) in group A. The incidence of chromosomal abnormalities in blastocysts from group B was significantly lower than group A blastocysts (8% (3/40) and 31% (20/64) respectively; P < 0.05). These data show that in-vitro cultured embryos had a significantly higher incidence of mosaicisim in comparison with the in-vivo group. (C) 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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