Zinc concentrations in serum and follicular fluid during ovarian stimulation and expression of Zn2+ transporters in human oocytes and cumulus cells

REPRODUCTIVE BIOMEDICINE ONLINE

卷:22

Article

Menezo, Yves^1,2  Pluntz, Laurene¹  Chouteau, Jacques³  Gurgan, Timur⁴  Demirol, Aygul⁴  Dalleac, Alain^1,2  Benkhalifa, Moncef¹ 

[1] UNILABS Lab Eylau, F-75116 Paris, France

[2] UNILABS Lab Oratoire Dynabio, Cherbourg, France

[3] Oriade, F-38000 Grenoble, France

[4] Gurgan Clin, Ankara, Turkey

关键词: cumulus cells;    follicular fluid;    metallothioneins;    oocyte;    serum;    zinc;   

DOI  :  10.1016/j.rbmo.2011.03.015

来源: Elsevier

【 摘 要 】

Serum and follicular fluid zinc concentrations were investigated in patients undergoing assisted reproductive treatment. No correlation was found between zinc and oestradiol concentrations in serum. At the time of oocyte retrieval, zinc concentrations in follicular fluid were significantly lower than serum concentrations (P < 0.0001). The expression of the two families of zinc transporters, ZnT and ZiP, as well as the metal regulatory transcription factors, MTF1 and 2, and metallothioneins, which are both involved in regulatory aspects of zinc transport, was assayed in cumulus cells and in germinal-vesicle oocytes. Most of the zinc transporters, metallothioneins and metal regulatory transcription factor are expressed in oocytes and not in cumulus cells. This may indicate an important role for zinc, in particular with potential linking to genome stability during early embryonic development. In contrast, cumulus cells seem to be at the end of their life's journey, with weak expression of transcriptional activity linked to cellular housekeeping. (C) 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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