Down-regulation of the CYP19A1 gene in cumulus cells of infertile women with endometriosis

REPRODUCTIVE BIOMEDICINE ONLINE

卷:30

Article

Barcelos, Ionara Diniz E. S.¹  Donabella, Flavia Capello¹  Ribas, Cristiana Padovan¹  Meola, Juliana¹  Ferriani, Rui Alberto¹  Paro de Paz, Claudia Cristina²  Navarro, Paula A.¹ 

[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Gynecol & Obstet, Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Genet, Ribeirao Preto, SP, Brazil

关键词: cumulus cells;    CYP19A1 gene;    endometriosis;    follicular fluid;    infertility;   

DOI  :  10.1016/j.rbmo.2015.01.012

来源: Elsevier

【 摘 要 】

Aromatase plays a fundamental role in the establishment of oocyte quality, which might be compromised in infertile women with endometriosis. The expression of the CYP19A1 gene (that encodes aromatase) was compared in cumulus cells and oestradiol concentrations in the follicular fluid of infertile women with and without endometriosis submitted to ovarian stimulation for intracytoplasmic sperm injection. Cumulus cells were isolated and the expression of the CYP19A1 was quantitated through real-time polymerase chain reaction. Oestradiol concentrations in follicular fluid were measured by chemiluminescence immunoassay. A lower expression of the CYP19A1 in the cumulus cells of infertile women with endometriosis was observed compared with controls (0.17 +/- 0.13 and 0.56 +/- 0.12, respectively), and no significant difference in the follicular fluid oestradiol concentrations was observed between groups. Our results show reduced expression of the CYP19A1 in cumulus cells of infertile women with endometriosis, which may play a role in the pathogenesis of endometriosis-related infertility. (C) 2015 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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