Improved development of mouse and human embryos using a tilting embryo culture system

REPRODUCTIVE BIOMEDICINE ONLINE

卷:20

Article

Matsuura, Koji^1,2  Hayashi, Nobuyoshi³  Kuroda, Yuka^1,2  Takiue, Chisato³  Hirata, Rei³  Takenami, Mami¹  Aoi, Yoko³  Yoshioka, Nanako³  Habara, Toshihiro³  Mukaida, Tetsunori⁴  Naruse, Keiji¹ 

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008530, Japan

[2] Okayama Univ, Res Core Int Erdisciplinary Sci, Okayama 7008530, Japan

[3] Okayama Couples Clin, Okayama, Japan

[4] Hiroshima HART Clin, Hiroshima, Japan

关键词: blastocyst;    embryo development;    mechanical stimuli;    shear stress;    tilting embryo culture system;   

DOI  :  10.1016/j.rbmo.2009.12.002

来源: Elsevier

【 摘 要 】

Mammalian embryos experience not only hormonal but also mechanical stimuli, such as shear stress, compression and friction force in the Fallopian tube before nidation. In order to apply mechanical stimuli to embryos in a conventional IVF culture system, the tilting embryo culture system (TECS) was developed. The observed embryo images from the TECS suggest that the velocities and shear stresses of TECS embryos are similar to those experienced in the oviduct. Use of TECS enhanced the development rate to the blastocyst stage and significantly increased the cell number of mouse blastocysts (P < 0.05). Although not statistically significant, human thawed embryos showed slight improvement in development to the blastocyst stage following culture in TECS compared with static controls. Rates of blastocyst formation following culture in TECS were significantly improved in low-quality embryos and those embryos cultured under suboptimal conditions (P < 0.05). The TECS is proposed as a promising approach to improve embryo development and blastocyst formation by exposing embryos to mechanical stimuli similar to those in the Fallopian tube. (C) 2009, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

Free