Live birth after artificial oocyte activation using a ready-to-use ionophore: a prospective multicentre study

REPRODUCTIVE BIOMEDICINE ONLINE

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Article

Ebner, Thomas¹  Montag, Markus³  Montag, M.³  Van der Ven, K.³  Van der Ven, H.³  Ebner, T.^1,2  Shebl, O.^1,2  Oppelt, P.^1,2  Hirchenhain, J.⁴  Kruessel, J.⁴  Maxrath, B.⁵  Gnoth, C.⁵  Friol, K.⁵  Tigges, J.⁵  Wuensch, E.⁶  Luckhaus, J.⁶  Beerkotte, A.⁶  Weiss, D.⁷  Grunwald, K.⁷  Struller, D.⁸  Etien, C.⁸ 

[1] Landes Frauen & Kinderklin, Dept Gynecol Endocrinol, Linz, Austria

[2] Landes Frauen & Kinderklin, Kinderwunsch Zentrum, Linz, Austria

[3] Univ Bonn, Dept Gynecol Endocrinol & Reprod Med, Bonn, Germany

[4] Univ Dusseldorf, UniKid, Dusseldorf, Germany

[5] Grevenbroicher Endokrinol & IVF Zentrum, Green IVF, Grevenbroich, Germany

[6] Inst Gynakol Endokrinol & Repodukt Med, Remscheid, Germany

[7] Ittertalklin, Aachen, Germany

[8] Kinderwunschpraxis Bedburg, Bedburg Erft, Germany

关键词: activation failure;    calcium;    fertilization;    ionophore A23187;    oocyte;    sperm;   

DOI  :  10.1016/j.rbmo.2014.11.012

来源: Elsevier

【 摘 要 】

Artificial oocyte activation has been proposed as a suitable means to overcome the problem of failed or impaired fertilization after intracytoplasmic sperm injection (ICSI). In a multicentre setting artificial oocyte activation was applied to 101 patients who were diagnosed with fertilization abnormalities (e.g. less than 50% fertilized oocytes) in a previous conventional ICSI cycle. Female gametes were activated for 15 min immediately after ICSI using a ready-to-use Ca2+-ionophore solution (A23187). Fertilization, pregnancy and live birth rates were compared with the preceding cycle without activation. The fertilization rate of 48% in the study cycles was significantly higher compared with the 25% in the control cycles (P < 0.001). Further splitting of the historical control group into failed (0%), low (1-30%) and moderate fertilization rate (31-50%) showed that all groups significantly benefitted (P < 0.001) in the ionophore cycle. Fewer patients had their embryo transfer cancelled compared with their previous treatments (1/101 versus 15/101). In total, 99% of the patients had an improved outcome with A23187 application resulting in a 28% live birth rate (35 babies). These data suggest that artificial oocyte activation using a ready-to-use compound is an efficient method. (C) 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

Free