Egg Activation at Fertilization: Where It All Begins☆

Developmental Biology

Linda L. Runft¹  Lisa M. Mehlmann¹  Laurinda A. Jaffe¹ 

[1] Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut, 06030

关键词: fertilization;    calcium;    egg activation;   

DOI  :  10.1006/dbio.2002.0600

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

AcentrallyimportantfactorininitiatingeggactivationatfertilizationisariseinfreeCa2+intheeggcytosol.Inechinoderm,ascidian,andvertebrateeggs,theCa2+riseoccursasaresultofinositoltrisphosphate-mediatedreleaseofCa2+fromtheendoplasmicreticulum.ThereleaseofCa2+atfertilizationinechinodermandascidianeggsrequiresSH2domain-mediatedactivationofaSrcfamilykinase(SFK)andphospholipaseC(PLC)γ.ThoughsomeevidenceindicatesthataSFKandPLCmayalsofunctionatfertilizationinvertebrateeggs,SH2domain-mediatedactivationofPLCγappearsnottoberequired.Muchworkhasfocusedonidentifyingfactorsfromspermthatinitiateeggactivationatfertilization,eitherasaresultofsperm–eggcontactorsperm–eggfusion.Currentevidencefromstudiesofascidianandmammalianfertilizationfavorsafusion-mediatedmechanism;thisissupportedbyexperimentsindicatingthatinjectionofspermextractsintoeggscausesCa2+releasebythesamepathwayasfertilization.

【 授权许可】

Unknown