Polymorphism of CAG and GGN repeats of androgen receptor gene in women with polycystic ovary syndrome

REPRODUCTIVE BIOMEDICINE ONLINE

卷:31

Article

Yuan, Chun¹  Gao, Chao¹  Qian, Yi¹  Liu, Ying¹  Jiang, Shi-Wen²  Cui, Yugui¹  Liu, Jiayin¹ 

[1] Nanjing Med Univ, Affiliated Hosp 1, Clin Ctr Reprod Med, State Key Lab Reprod Med, Nanjing, Jiangsu, Peoples R China

[2] Mercer Univ, Sch Med, Dept Biomed Sci, Macon, GA USA

关键词: androgen receptor;    endocrinology;    hyperandrogenism;    polycystic ovary syndrome;    polymorphism;   

DOI  :  10.1016/j.rbmo.2015.09.007

来源: Elsevier

【 摘 要 】

One characteristic of polycystic ovary syndrome (PCOS) is hyperandrogenism, which may be related to the activity of androgen receptor (AR). This study was designed to investigate the polymorphism of CAG and GGN repeats in the AR gene in women with PCOS. The frequency distributions of CAG and GGN repeat alleles, as well as their X-inactivation patterns, were compared between 76 age-matched normal women (control group) and 80 women with PCOS (PCOS group). The expression of AR mRNA in the ovarian tissues of seven patients with PCOS and five normal women was also tested using real-time quantitative PCR. It was found that PCOS patients had significantly higher frequency of longer GGN biallelic mean (29.8%) and X-weighted biallelic mean (33.3%) than controls (6.1% and 3.2%, respectively, P = 0.002, P = 0.003). The odds ratio of the long GGN repeat length (n > 16) before and after X-chromosome inactivation (XCI) in the PCOS group was significantly higher than in controls (P = 0.0001, P = 0.005). AR-GGN repeat mRNA expression was higher in the ovarian tissue of controls compared with PCOS patients (P = 0.022). In conclusion, the data suggest that the GGN repeat polymorphism in the AR gene is associated with PCOS. (C) 2015 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

Free