期刊论文详细信息
REPRODUCTIVE BIOMEDICINE ONLINE 卷:24
Peritoneal fluid modifies the response of human spermatozoa to follicular fluid
Article
Jose Munuce, Maria1 
[1] Univ Nacl Rosario, Sch Biochem & Pharmaceut Sci, Dept Clin Biochem, RA-2000 Rosario, Santa Fe, Argentina
关键词: acrosome reaction;    capacitation;    follicular fluid;    human spermatozoa;    peritoneal fluid;    progesterone;   
DOI  :  10.1016/j.rbmo.2011.12.010
来源: Elsevier
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【 摘 要 】

The aim of this study was to elucidate the mechanism involved in the acrosome reaction (AR) induced by follicular fluid (FF) in spermatozoa previously exposed to peritoneal fluid (PF). The influence of progesterone was also investigated. Semen samples were from 18 normozoospermic donors. PF samples were from 13 women with unexplained infertility and from a woman treated with synthetic progestagen. FF samples were collected from six women undergoing IVF/embryo transfer and pooled. Motile spermatozoa were capacitated overnight and a kinetic and inhibition study on the FF-induced AR was performed. Spermatozoa pretreated with PF were challenged with either FF or progesterone. The ability of progesterone-and progestagen-supplemented PF to induce AR was analysed. Enzyme-digested PF was also tested. Pre-incubation with PF for 60 min completely prevented the FF-induced AR; spermatozoa treated with PF were unable to respond to FF or progesterone and this effect was not reversible. Progesterone-and progestagen-supplemented PF stimulated the AR relative to controls. Enzyme-digested PF did not have an inhibitory capacity. These data strongly suggest that there are one or more inhibitory proteins in PF that interact with spermatozoa so as to prevent access of progesterone to its receptor and thus inhibit the occurrence of the AR. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

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