Preparation of a sugarcane bagasse-based substrate for second-generation ethanol: Effect of pasteurisation conditions on dephenolisation

RENEWABLE ENERGY

卷:157

Article

Gonzalez-Bautista, Enrique^1,2  Alarcon-Gutierrez, Enrique²  Dupuy, Nathalie¹  Gaime-Perraud, Isabelle¹  Ziarelli, Fabio³  Foli, Lisa¹  Farnet-Da-Silva, Anne-Marie¹ 

[1] Avignon Univ, Aix Marseille Univ, CNRS, IRD,IMBE,UMR 7263, Marseille, France

[2] Univ Veracruzana, Inst Biotecnol & Ecol Aplicada INBIOTECA, Campus Cultura,Artes & Deporte, Xalapa 91090, Veracruz, Mexico

[3] Aix Marseille Univ, CNRS, Fed Sci Chim Marseille, FR 1739, Spectropole Campus St Jerome, F-13397 Marseille, France

关键词: Heat pre-treatment;    Lignocellulose transformation;    Solid-state fermentation;    White-rot fungi;    Laccase;   

DOI  :  10.1016/j.renene.2020.05.116

来源: Elsevier

【 摘 要 】

Second-generation ethanol process uses lignocellulose, but a pre-treatment is required to degrade lignin before saccharification. Biological pre-treatment, using phenoloxidases, represents a cheap and eco-friendly option. Mould contamination can be overcome by heat pre-conditioning of substrates. Pasteurisation can be a mild and sustainable option. A 5-level response-surface experimental design was performed to test the effect of different time and temperatures on lignocellulolytic activities and substrate dephenolisation. Substrate microbial communities were characterised via catabolic profiles to determine functional diversity changes after pasteurisation. Temperatures ranging from 70 to 75 degrees C and time from 5 to 10 h led to the highest laccase activities and 30% of substrate dephenolisation was achieved while avoiding mould contamination. The per cent of O-Alkyl (polysaccharides) did not vary significantly, meaning that polysaccharides were not extensively consumed. Microbial communities were less catabolically diversified (H' = 1.2) but more active (AWCD = 0.9) and could degrade polysaccharides. (C) 2020 Published by Elsevier Ltd.

【 授权许可】

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