Bioethanol production from sweet potato by co-immobilization of saccharolytic molds and Saccharomyces cerevisiae

RENEWABLE ENERGY

卷:39

Article

Lee, Wen-Shiang^1,2  Chen, I-Chu^1,2  Chang, Cheng-Hsiung^1,2  Yang, Shang-Shyng^1,2 

[1] China Univ Sci & Technol, Dept Food Sci, Taipei 11581, Taiwan

[2] Natl Taiwan Univ, Dept Biochem Sci & Technol, Taipei 10617, Taiwan

关键词: Co-immobilization;    Bioethanol;    Sweet potato;    Aspergillus oryzae;    Monascus purpureus;    Saccharomyces cerevisiae;   

DOI  :  10.1016/j.renene.2011.08.024

来源: Elsevier

【 摘 要 】

To investigate the bioethanol production from sweet potato, the saccharification and fermentation conditions of co-immobilization of saccharolytic molds (Aspergillus oryzae and Monascus purpureus) with Saccharomyces cerevisiae were analyzed. The immobilized yeast cells showed that at 10% glucose YPD (yeast extract peptone dextrose) the maximum fermentation rate was 80.23%. Viability of yeasts cells were 95.70% at a final ethanol concentration of 6%. Immobilization enhanced the ethanol tolerance of yeast cells. In co-immobilization of S. cerevisiae with A. oryzae or M. purpureus, the optimal hardening time of gel beads was between 15 and 60 min. Bioethanol production was 3.05-3.17% (v v(-1)) and the Y-E/S (yield of ethanol production/starch consumption) was 0.31-0.37 at pH 4,30 degrees C and 150 rpm during 13 days fermentation period. Co-immobilization of S. cerevisiae with a mixed cultures of A. oryzae and M. purpureus at a ratio of 2:1, the bioethanol production was 3.84% (v v(-1)), and the Y-E/s was 0.39 for a 11 days incubation. However a ratio of A. oryzae and M. purpureus at 1:2 resulted a bioethanol production rate of 4.08% (v v(-1)), and a Y-E/s of 0.41 after 9 days of fermentation. (C) 2011 Elsevier Ltd. All rights reserved.

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