The Influence of Non-Nociceptive Factors on Hot-Plate Latency in Rats

JOURNAL OF PAIN

卷:12

Article

Gunn, Amanda¹  Bobeck, Erin N.¹  Weber, Ceri¹  Morgan, Michael M.¹ 

[1] Washington State Univ Vancouver, Dept Psychol, Vancouver, WA 98686 USA

关键词: Pain;    nociception;    sex differences;    hyperalgesia;    morphine;   

DOI  :  10.1016/j.jpain.2010.06.011

来源: Elsevier

【 摘 要 】

The hot plate is a widely used test to assess nociception. The effect of non-nociceptive factors (weight, sex, activity, habituation, and repeated testing) on hot-plate latency was examined. Comparison of body weight and hot-plate latency revealed a small but significant inverse correlation (light rats had longer latencies). Habituating rats to the test room for 1 hour prior to testing did not decrease hot-plate latency except for female rats tested on days 2 to 4. Hot-plate latency decreased with repeated daily testing, but this was not caused by a decrease in locomotor activity or learning to respond. Activity on the hot plate was consistent across all 4 trials, and prior exposure to a room-temperature plate caused a similar decrease in latency as rats tested repeatedly on the hot plate. Despite this decrease in baseline hot-plate latency, there was no difference in morphine antinociceptive potency. The present study shows that weight, habituation to the test room, and repeated testing can alter baseline hot-plate latency, but these effects are small and have relatively little impact on morphine antinociception. Perspective: This manuscript shows that non-nociceptive factors such as body weight, habituation, and repeated testing can alter hot-plate latency, but these factors do not alter morphine potency. In sum, the hot-plate test is an easy to use and reliable method to assess supraspinally organized nociceptive responses. (C) 2011 by the American Pain Society

【 授权许可】

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