Abnormal pulmonary endothelial cells may underlie the enigmatic pathogenesis of chronic thromboembolic pulmonary hypertension

JOURNAL OF HEART AND LUNG TRANSPLANTATION

卷:36

Article

Mercier, Olaf^1,2,3  Ataam, Jennifer Arthur¹  Langer, Nathaniel. B.^1,2,3  Dorfmueller, Peter^1,4  Lamrani, Lilia¹  Lecerf, Florence¹  Decante, Benoit¹  Dartevelle, Philippe^1,2,3  Eddahibi, Saadia^1,5  Fadel, Elie^1,2,3 

[1] Univ Paris Sud, Marie Lannelongue Hosp, Res & Innovat Unit, INSERM U999,DHU TORINO, Le Plessis Robinson, France

[2] Marie Lannelongue Hosp, Dept Thorac & Vasc Surg, Le Plessis Robinson, France

[3] Marie Lannelongue Hosp, Dept Heart Lung Transplantat, Le Plessis Robinson, France

[4] Marie Lannelongue Hosp, Pathol, Le Plessis Robinson, France

[5] Univ Montpellier, CHU Arnaud Villeneuve Montpellier, CNRS UMR 9214, INSERM U1046, Montpellier, France

关键词: chronic thromboembolic pulmonary hypertension;    venous thromboembolism;    pulmonary artery endothelial cells;    growth factors;    cytokine signaling;    adhesion molecules;    pathogenesis;   

DOI  :  10.1016/j.healun.2016.08.012

来源: Elsevier

【 摘 要 】

BACKGROUND: Chronic thromboembolic pulmonary hypertension results from chronic mechanical obstruction of the pulmonary arteries after acute venous-thromboembolism. However, the mechanisms that result in the progression from unresolved thrombus to fibrotic vascular remodeling are unknown. We hypothesized that pulmonary artery endothelial cells contribute to this phenomenon via paracrine growth factor and cytokine signaling. METHODS: Using enzyme-linked immunosorbent assay and cell migration assays, we investigated the circulating growth factors and cytokines of chronic thromboembolic pulmonary hypertension patients as well as the cross talk between pulmonary endothelial cells and pulmonary artery smooth muscle cells and monocytes from patients with chronic thromboembolic pulmonary hypertension in vitro. RESULTS: Culture medium from the pulmonary endothelial cells of chronic thromboembolic pulmonary hypertension patients contained higher levels of growth factors (fibroblast growth factor 2), inflammatory cytokines (interleukin 1 beta, interleukin 6, monocyte chemoattractant protein 1), and cell adhesion molecules (vascular cell adhesion molecule 1 and intercellular adhesion molecule 1). Furthermore, exposure to the culture medium of pulmonary endothelial cells from patients with chronic thromboembolic pulmonary hypertension elicited marked pulmonary artery smooth muscle cell growth and monocyte migration. CONCLUSIONS: These findings implicate pulmonary endothelial cells as key regulators of pulmonary artery smooth muscle cell and monocyte behavior in chronic thromboembolic pulmonary hypertension and suggest a potential mechanism for the progression from unresolved thrombus to fibrotic vascular remodeling. (C) 2017 International Society for Heart and Lung Transplantation. All rights reserved.

【 授权许可】

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