Protein microarray using α-amino acids as metal tags on chips

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:15

Article

Sinchaikul, S ; Pan, FM ; Cheng, CW ; Wong, CH ; Fang, JM ; Tseng, MJ ; Chen, ST

关键词: protein microarray;    protein chip;    metal tag;    3CL-protease;   

DOI  :  10.1016/j.bmcl.2005.01.001

来源: Elsevier

【 摘 要 】

Procedures for synthesizing alpha-amino acids on a chip for coordination with transitional metal ions and a His-Tagged protein have been successfully developed as a stable protein microarray. Using the recombinant His-Tagged 3CL-protease (3CL(pro)) as a model for attachment to chips containing D-/L-Glu, Asp, Orn, Set via different transitional metal ions, it was found that the Orn chip was the best of affinity binding and stability by which Zn2+ was the best metal ion for affinity while Co2+ was the best metal ion for stability. Thus, this protein microarray can be alternatively used as a high throughput screening method for rapid detection against SARS CoV 3CL(pro) and/or efficient purification of other Tagged proteins. (c) 2005 Elsevier Ltd. All rights reserved.

【 授权许可】

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