Serodiagnostic Comparison between Two Methods, ELISA and Surface Plasmon Resonance for the Detection of Antibodies of Classical Swine Fever

Journal of Veterinary Medical Science

Nam-Yong PARK¹  Ho-Seong CHO¹ 

[1] Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University

关键词: classical swine fever;    protein chip;    surface plasmon resonance;   

DOI  :  10.1292/jvms.68.1327

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(10)A protein chip based on surface plasmon resonance (SPR) was develpoped to measure the antibody (Ab) titers of classical swine fever virus (CSFV) using the recombinant gp55 protein as an antigen. The diagnostic potential of this SPR assay for detecting the Ab titers to CSFV gp55 was compared that of the enzyme-linked immunosorbent assay (ELISA) using 170 serum samples from 14 pig farms. The SPR assay was highly specific and sensitive, and there were no cross-reactions detected. There was a strong positive correlation between the SPR and ELISA titers (n=170, r=0.869, p<0.01). Therefore, the SPR label-free method is a valuable tool in the serodiagnosis of CSFV infection and determining Ab titers after vaccination.

【 授权许可】

Unknown