Glycan array analysis of the antigen repertoire targeted by tumor-binding antibodies

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:22

Article

Gildersleeve, Jeffrey C.¹  Wang, Baomei²  Achilefu, Samuel³  Tu, Zhude³  Xu, Mai³ 

[1] NCI, Biol Chem Lab, Frederick, MD 21702 USA

[2] Univ Louisville, James Graham Brown Canc Ctr, Dept Microbiol & Immunol, Louisville, KY 40202 USA

[3] Washington Univ, Sch Med, Dept Radiol, St Louis, MO 63110 USA

关键词: Glycan array;    Antibody;    Carbohydrate;    Tumor antigen;    Cancer vaccine;   

DOI  :  10.1016/j.bmcl.2012.09.055

来源: Elsevier

【 摘 要 】

Immunization with whole cells has been used extensively to generate monoclonal antibodies, produce protective immune responses, and discover new disease antigens. While glycans are abundant on cell surfaces, anti-glycan immune responses have not been well-characterized. We used glycan microarrays to profile 49 tumor-binding monoclonal antibodies generated by immunizing mice with whole cancer cells. A substantial proportion (41%) of the tumor binding antibodies bound carbohydrate antigens. The antibodies primarily recognize a group of 5 glycan antigens: Sialyl Lewis A (SLeA), Lewis A (LeA), Lewis X (LeX), blood group A (BG-A), and blood group H on a type 2 chain (BG-H2). The results have important implications for monoclonal antibody production and cancer vaccine development. Published by Elsevier Ltd.

【 授权许可】

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