Assessment of glycan interactions of clinical and avian isolates of Campylobacter jejuni

BMC Microbiology

Research Article

Greg Tram¹  Lauren E Hartley-Tassell¹  Christopher J Day¹  Joe Tiralongo¹  Victoria Korolik¹ 

[1] Institute for Glycomics, G26, Griffith University Gold Coast Campus, 4222, Queensland, Australia;

关键词: Host-bacterial interactions;    Glycan array;    Glycoconjugates;   

DOI  :  10.1186/1471-2180-13-228

 received in 2013-07-29, accepted in 2013-09-27,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundCampylobacter jejuni strain 11168 was demonstrated to have a broad specificity for eukaryotic surface glycosylation using glycan array analysis. The initial screen indicated that sialic acid and mannose are important binding partners after environmental stress, while galactose and fucose structures are likely to be involved in persistent infection.ResultsIn this broader study, five additional human/clinical isolates and six chicken isolates were fully assessed to determine their glycan binding capacity using an extended glycan array. C. jejuni 11168 was rescreened here due to the presence of glycoaminoglycan (GAG) and other structures that were not available on our previous glycan array. The current array analysis of additional C. jejuni strains confirmed the growth condition dependent differences in glycan binding that was previously observed for C. jejuni 11168. We noted strain to strain variations, particularly for the human isolates C. jejuni 520 and 81116 and the chicken isolate C. jejuni 331, with the majority of differences observed in galactose, mannose and GAG binding. Chicken isolates were found to bind to a broader range of glycans compared to the human isolates, recognising branched mannose and carageenan (red seaweed) glycans. Glycan array data was confirmed using cell-based lectin inhibition assays with the fucose (UEA-I) and mannose (ConA) binding lectins.ConclusionsThis study confirms that all C. jejuni strains tested bind to a broad range of glycans, with the majority of strains (all except 81116) altering recognition of sialic acid and mannose after environmental stress. Galactose and fucose structures were bound best by all strains when C. jejuni was grown under host like conditions confirming the likelihood of these structures being involved in persistent infection.

【 授权许可】

Unknown   
© Day et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.